A new, trouble-free, economic, reproducible and accurate stability indicating assay method using UV-Visible spectrophotometer for the quantitative evaluation of Tinidazole in bulk and pharmaceutical dosage forms has been developed. 0.1 N HCl was chosen as the solvent system for developing assay method. The absorbance maximum (λmax) for Tinidazole was found to be 277 nm. The responses were linear in the range of 10-80 μg/ml. The regression equation of the calibration graph and correlation coefficient were found to be y = 0.030 x + 0.022 and 0.999 respectively. The method was validated statistically and found to be accurate with 99.71±0.11203 % recovery), precise at intraday (0.0923 %RSD) and inter day (0.4825 %RSD), specific (99.86 ± 0.076 %). LOD and LOQ were found to be 0.04 μg/ml and 0.1 μg/ml respectively. This method was precisely determining the concentration of Tinidazole in marketed brand namely Tiniba 300 and the results were in good agreement with the label claim.
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